Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Mmu Input
strain
C57BL/6JOlaHsd
developmental stage
E9.5 + E14.5
cell type
pooled

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the LowCell ChIP kit (Diagenode) and DNA was purified using the IPure kit (Diagenode) according to manufacturer’s instructions. DNA was subjected to NGS library preparation using the MicroPlex Template Preparation Kit from Diagenode.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
16652919
Reads aligned (%)
77.0
Duplicates removed (%)
18.0
Number of peaks
271 (qval < 1E-05)

mm9

Number of total reads
16652919
Reads aligned (%)
76.8
Duplicates removed (%)
18.1
Number of peaks
230 (qval < 1E-05)

Base call quality data from DBCLS SRA