Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
Neuroepithelial Cells
MeSH Description
Cells of epithelial origin possessing specialized sensory functions. They include cells that are found in the TASTE BUDS; OLFACTORY MUCOSA; COCHLEA; and NEUROEPITHELIAL BODIES.

Attributes by original data submitter

Sample

source_name
Mmu NEC H3K4me3
strain
C57BL/6JOlaHsd
developmental stage
E9.5
cell type
neuroepithelial cell
chip antibody
anti-H3K4me3 (Cell Signaling, 9751S, LC42,D8, Lot 7)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the LowCell ChIP kit (Diagenode) and DNA was purified using the IPure kit (Diagenode) according to manufacturer’s instructions. DNA was subjected to NGS library preparation using the MicroPlex Template Preparation Kit from Diagenode.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
47751935
Reads aligned (%)
98.2
Duplicates removed (%)
87.8
Number of peaks
8771 (qval < 1E-05)

mm9

Number of total reads
47751935
Reads aligned (%)
98.1
Duplicates removed (%)
87.9
Number of peaks
8779 (qval < 1E-05)

Base call quality data from DBCLS SRA