Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Pre-B ALL
NA
NA

Attributes by original data submitter

Sample

source_name
Ik6 pre-B ALL + TET-Ik1
cell-line
in vitro growing pre-B ALL xenograft-derived cells
genotype/variation
Ik6,BCR-ABL1
induction time
48 hour
antibody
H3K27ac
treatment
IK1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. According to the NEBNext ChIP-seq protocol, with 12-15 cycles of PCR.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
23802762
Reads aligned (%)
98.7
Duplicates removed (%)
3.0
Number of peaks
1955 (qval < 1E-05)

hg19

Number of total reads
23802762
Reads aligned (%)
97.8
Duplicates removed (%)
4.4
Number of peaks
1747 (qval < 1E-05)

Base call quality data from DBCLS SRA