GSM2410102: Ik1 H3K27ac rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
Pre-B ALL
NA
NA
Attributes by original data submitter
Sample
source_name
Ik6 pre-B ALL + TET-Ik1
cell-line
in vitro growing pre-B ALL xenograft-derived cells
genotype/variation
Ik6,BCR-ABL1
induction time
48 hour
antibody
H3K27ac
treatment
IK1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. According to the NEBNext ChIP-seq protocol, with 12-15 cycles of PCR.