Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Hematopoietic Stem Cells

Cell type information


MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by Original Data Submitter


source_name
peripheral blood
tissue
peripheral blood
cell type
hematopoietic stem cell (HSCs)
antibody
anti-H3K4Me2, Abcam ab32356

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Human peripheral blood mononuclear cells (PBMC) from peripheral blood were isolated by Ficoll-Paque density gradient centrifugation. Cells were isolated by FACS (ILCP: viable CD45+ Lin- (CD3- CD4- CD5- TCRαβ- TCRγδ- CD14- CD19-) CD7+ CD127+ CRTh2- CD117+ cells, HSC: CD45+ CD34+) Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015).

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
47553519
Reads aligned (%)
95.8
Duplicates removed (%)
14.5
Number of peaks
35351 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA