Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Lymphoid Progenitor Cells

MeSH Description

Stem cells from which B-LYMPHOCYTES; T-LYMPHOCYTES; NATURAL KILLER CELLS; and some DENDRITIC CELLS derive.

Sample

source_name

peripheral blood

tissue

peripheral blood

cell type

Innate lymphoid cell precursors (ILCPs)

antibody

anti-H3K4Me2, Abcam ab32356

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Human peripheral blood mononuclear cells (PBMC) from peripheral blood were isolated by Ficoll-Paque density gradient centrifugation. Cells were isolated by FACS (ILCP: viable CD45+ Lin- (CD3- CD4- CD5- TCRαβ- TCRγδ- CD14- CD19-) CD7+ CD127+ CRTh2- CD117+ cells, HSC: CD45+ CD34+) Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

34531145

Reads aligned (%)

94.3

Duplicates removed (%)

17.7

Number of peaks

2734 (qval < 1E-05)

hg19

Number of total reads

34531145

Reads aligned (%)

91.6

Duplicates removed (%)

17.5

Number of peaks

3666 (qval < 1E-05)