Antigen

Antigen Class

TFs and others

Antigen

AHR

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7 breast cancer cells

cell type

epithelial cells

tissue

mammary gland, breast

chip-antibody

anti-AHR (Santa Cruz, H-211)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were sonicated using Diagenode Bioruptor sonicator and protein-DNA complexes were isolated with antibody and beads MicroPlex Library Preparation Kit using the manufacturer recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

71291875

Reads aligned (%)

99.0

Duplicates removed (%)

33.1

Number of peaks

23343 (qval < 1E-05)

hg19

Number of total reads

71291875

Reads aligned (%)

98.4

Duplicates removed (%)

34.6

Number of peaks

23221 (qval < 1E-05)