Antigen

Antigen Class

TFs and others

Antigen

AHR

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7 breast cancer cells

cell type

epithelial cells

tissue

mammary gland, breast

chip-antibody

anti-AHR (Santa Cruz, H-211)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were sonicated using Diagenode Bioruptor sonicator and protein-DNA complexes were isolated with antibody and beads MicroPlex Library Preparation Kit using the manufacturer recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

72723927

Reads aligned (%)

98.3

Duplicates removed (%)

51.8

Number of peaks

5391 (qval < 1E-05)

hg19

Number of total reads

72723927

Reads aligned (%)

97.7

Duplicates removed (%)

53.5

Number of peaks

5392 (qval < 1E-05)