Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AHR

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cells
cell type
epithelial cells
tissue
mammary gland, breast
chip-antibody
anti-AHR (Santa Cruz, H-211)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sonicated using Diagenode Bioruptor sonicator and protein-DNA complexes were isolated with antibody and beads MicroPlex Library Preparation Kit using the manufacturer recommended protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
18437120
Reads aligned (%)
156.3
Duplicates removed (%)
15.0
Number of peaks
3696 (qval < 1E-05)

hg38

Number of total reads
18437120
Reads aligned (%)
158.4
Duplicates removed (%)
14.6
Number of peaks
4119 (qval < 1E-05)

Base call quality data from DBCLS SRA