Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AFF4

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input
mv411_input_Rx
treatment
0.01% DMSO, 500 nM dTAG-13 for 6 hr
sequencing barcode
TGAACTGG
antibody
AFF4
antibody manufacturer
Abcam
antibody catalog number
Ab57077

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
96348892
Reads aligned (%)
6.5
Duplicates removed (%)
90.4
Number of peaks
419 (qval < 1E-05)

hg38

Number of total reads
96348892
Reads aligned (%)
7.4
Duplicates removed (%)
89.1
Number of peaks
1221 (qval < 1E-05)

Base call quality data from DBCLS SRA