Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input
mv411_input_Rx
treatment
0.01% DMSO for 24 hr
sequencing barcode
TAGTCGTG
antibody
RNA Pol II
antibody manufacturer
Diagenode
antibody catalog number
C15100055

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
95850214
Reads aligned (%)
71.9
Duplicates removed (%)
26.7
Number of peaks
28186 (qval < 1E-05)

hg19

Number of total reads
95850214
Reads aligned (%)
71.2
Duplicates removed (%)
26.7
Number of peaks
27815 (qval < 1E-05)

Base call quality data from DBCLS SRA