Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K18ac

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input
mv411_input
treatment
none
sequencing barcode
GTGCTACC
antibody
H3K18ac
antibody manufacturer
Millipore
antibody catalog number
07-354

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
39193096
Reads aligned (%)
97.0
Duplicates removed (%)
27.1
Number of peaks
51097 (qval < 1E-05)

hg19

Number of total reads
39193096
Reads aligned (%)
96.7
Duplicates removed (%)
27.2
Number of peaks
51107 (qval < 1E-05)

Base call quality data from DBCLS SRA