GSM2402810: SW480 WT TAZ Sigma; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
Micrococcal nuclease treated chromatin was pulled down with TAZ antibody. The MicroPlex Library Preparation Kit v2 (Diagenode) was used to prepare indexed libraries from 1ng of ChIP DNA. The resulting libraries were then size-selected (selecting a 200-800bp range) by adding 0.55X volume of AMPure XP beads (Beckman Coulter). To the supernatant, 0.3X the original sample volume of AMPure XP beads were added. The supernatant was discarded and the beads washed with 70% ethanol before drying and eluting the size-selected library. Library quality was checked using the Agilent Bioanalyzer. Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina (Kapa Biosystems Inc.). 1.7 pM pooled libraries were loaded onto the NextSeq 500 and 2x75bp sequencing was carried out using a NextSeq 500/550 High Output v2 kit (Illumina Inc.).