GSM2402808: SW480 TIAM1 KO TAZ Sigma; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TAZ
Cell type
Cell type Class
Digestive tract
Cell type
SW 480
Primary Tissue
unresolved
Tissue Diagnosis
unresolved
Attributes by original data submitter
Sample
source_name
SW480_TIAM1 knockout
cell line
SW480
source tissue
colon
genotype/variation
TIAM1 knockout
chip antibody
TAZ antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Micrococcal nuclease treated chromatin was pulled down with TAZ antibody. The MicroPlex Library Preparation Kit v2 (Diagenode) was used to prepare indexed libraries from 1ng of ChIP DNA. The resulting libraries were then size-selected (selecting a 200-800bp range) by adding 0.55X volume of AMPure XP beads (Beckman Coulter). To the supernatant, 0.3X the original sample volume of AMPure XP beads were added. The supernatant was discarded and the beads washed with 70% ethanol before drying and eluting the size-selected library. Library quality was checked using the Agilent Bioanalyzer. Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina (Kapa Biosystems Inc.). 1.7 pM pooled libraries were loaded onto the NextSeq 500 and 2x75bp sequencing was carried out using a NextSeq 500/550 High Output v2 kit (Illumina Inc.).