Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA2

Cell type

Cell type Class
Epidermis
Cell type
Ker-CT
Tissue
Foreskin
Cell Type
Keratinocyte
Disease
Normal

Attributes by original data submitter

Sample

source_name
FOXA2 ChIP Keratenocytes
cell line
KerCT
chip antibody
FoxA2 Antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27111177
Reads aligned (%)
181.2
Duplicates removed (%)
7.7
Number of peaks
54944 (qval < 1E-05)

hg38

Number of total reads
27111177
Reads aligned (%)
182.2
Duplicates removed (%)
7.6
Number of peaks
54994 (qval < 1E-05)

Base call quality data from DBCLS SRA