Antigen

Antigen Class

TFs and others

Antigen

FOXA2

Cell type

Cell type Class

Epidermis

Cell type

Ker-CT

Tissue

Foreskin

Cell Type

Keratinocyte

Disease

Normal

Sample

source_name

FOXA2 ChIP Keratenocytes

cell line

KerCT

chip antibody

FoxA2 Antibody

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

27111177

Reads aligned (%)

98.6

Duplicates removed (%)

7.6

Number of peaks

54994 (qval < 1E-05)

hg19

Number of total reads

27111177

Reads aligned (%)

98.2

Duplicates removed (%)

7.7

Number of peaks

54944 (qval < 1E-05)