Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pancreas

Cell type

L3.6

NA

NA

Sample

source_name

L3.6_DMSO_H3K4me3

immortalization type

SV40 large T antigen immortalized

cell line

L3.6

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP was performed with 10 min crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1-2µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standard phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

16125609

Reads aligned (%)

95.0

Duplicates removed (%)

82.8

Number of peaks

17619 (qval < 1E-05)

hg19

Number of total reads

16125609

Reads aligned (%)

94.8

Duplicates removed (%)

83.1

Number of peaks

17425 (qval < 1E-05)