Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
L3.6
NA
NA

Attributes by original data submitter

Sample

source_name
L3.6_DMSO_input
immortalization type
SV40 large T antigen immortalized
cell line
L3.6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP was performed with 10 min crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1-2µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standard phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
16389605
Reads aligned (%)
78.2
Duplicates removed (%)
4.6
Number of peaks
1893 (qval < 1E-05)

hg19

Number of total reads
16389605
Reads aligned (%)
77.3
Duplicates removed (%)
5.0
Number of peaks
1774 (qval < 1E-05)

Base call quality data from DBCLS SRA