Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Phf8 KO_cortical neuronal chromatin_None
strain background
B6x129SvJ
genotype/variation
Phf8 KO
age
2mo
tissue
Neocortex
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Neuronal nuclei were FACS sorted from the neocorteces of WT and KO mice. Native chromatin was fragmented by MNase digest and ChIPed with the indicated antibody Libraries were constructed with the Illumina TruSeq ChIP prep kit following the manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
40215383
Reads aligned (%)
97.7
Duplicates removed (%)
9.6
Number of peaks
161 (qval < 1E-05)

mm9

Number of total reads
40215383
Reads aligned (%)
97.6
Duplicates removed (%)
10.0
Number of peaks
151 (qval < 1E-05)

Base call quality data from DBCLS SRA