Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
Mammary glands
NA
NA

Attributes by original data submitter

Sample

source_name
FACS-sorted cells from mouse mammary gland
sample
6
subpopulation
Lgr5-Tspan8hi
tissue
Mammary gland
antibody
none (input)
genetic background
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed and the chromatin was sheared to a size range of 200 to 400 bp using Covaris M220 sonicator. Sheared chromatin was diluted and incubated at 4°C overnight with antibodies against Histone H3 trimethyl Lys4 (K4, Millipore #07-473), Histone H3 trimethyl Lys27. ChIP samples were prepared and indexed for Illumina sequencing using the TruSeq DNA sample Prep Kit.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
28252419
Reads aligned (%)
95.3
Duplicates removed (%)
59.4
Number of peaks
101 (qval < 1E-05)

mm9

Number of total reads
28252419
Reads aligned (%)
95.1
Duplicates removed (%)
59.5
Number of peaks
77 (qval < 1E-05)

Base call quality data from DBCLS SRA