GSM2393843: LnCaP Bag1L KO Bag1L Rescue DHT 4h AR ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LnCaP Bag-1L KO cells plus Bag-1L
sample type
LnCaP Bag-1L KO cells plus Bag-1L
treatment
10 nM DHT (4 h)
chip antibody
AR (N-20) antibody (Santa Cruz, Catalog# sc-816, Lot# I0215)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked cells were lysed using a Sarkosyl-containing lysis buffer. Chromatin was isolated, sheared and subjected to immunoprecipitation with an anti-AR antibody. Libraries were prepared using 5 ng ChIP DNA and the ThruPLEX DNA-seq kit (Rubicon Genomics) according to the manufacturer's instructions.