Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

LnCaP control cells

sample type

LnCaP control cells

treatment

10 nM DHT (4 h)

chip antibody

AR (N-20) antibody (Santa Cruz, Catalog# sc-816, Lot# I0215)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked cells were lysed using a Sarkosyl-containing lysis buffer. Chromatin was isolated, sheared and subjected to immunoprecipitation with an anti-AR antibody. Libraries were prepared using 5 ng ChIP DNA and the ThruPLEX DNA-seq kit (Rubicon Genomics) according to the manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

83514418

Reads aligned (%)

89.5

Duplicates removed (%)

6.9

Number of peaks

35970 (qval < 1E-05)

hg38

Number of total reads

83514418

Reads aligned (%)

91.9

Duplicates removed (%)

5.4

Number of peaks

36105 (qval < 1E-05)