Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LnCaP control cells
sample type
LnCaP control cells
treatment
10 nM DHT (4 h)
chip antibody
AR (N-20) antibody (Santa Cruz, Catalog# sc-816, Lot# I0215)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked cells were lysed using a Sarkosyl-containing lysis buffer. Chromatin was isolated, sheared and subjected to immunoprecipitation with an anti-AR antibody. Libraries were prepared using 5 ng ChIP DNA and the ThruPLEX DNA-seq kit (Rubicon Genomics) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
83514418
Reads aligned (%)
89.5
Duplicates removed (%)
6.9
Number of peaks
35970 (qval < 1E-05)

hg38

Number of total reads
83514418
Reads aligned (%)
91.9
Duplicates removed (%)
5.4
Number of peaks
36105 (qval < 1E-05)

Base call quality data from DBCLS SRA