Antigen

Antigen Class

TFs and others

Antigen

Spi1

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

preGM

genotype

CEBPA KO

cell markers

Lin-, Sca-1-, ckit+, CD150-, CD105-

tissue

bone marrow

chip-antibody

Sc-352, Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA extraction protocol not provided. Precipitated DNA were mixed with 0-2 ng E. Coli DNA, depending on the amount of ChIP DNA isolated. This mix was amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturer’s protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

250680674

Reads aligned (%)

21.2

Duplicates removed (%)

61.6

Number of peaks

41465 (qval < 1E-05)

mm9

Number of total reads

250680674

Reads aligned (%)

21.1

Duplicates removed (%)

61.7

Number of peaks

41504 (qval < 1E-05)