Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
LSK cells
NA
NA

Attributes by original data submitter

Sample

source_name
LSK
genotype
wildtype
cell markers
Lin-, Sca-1+, ckit
tissue
bone marrow
chip-antibody
Sc-352, Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA extraction protocol not provided. Precipitated DNA were mixed with 0-2 ng E. Coli DNA, depending on the amount of ChIP DNA isolated. This mix was amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturer’s protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
245131763
Reads aligned (%)
23.6
Duplicates removed (%)
66.7
Number of peaks
36345 (qval < 1E-05)

mm9

Number of total reads
245131763
Reads aligned (%)
23.6
Duplicates removed (%)
66.8
Number of peaks
36427 (qval < 1E-05)

Base call quality data from DBCLS SRA