Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Treg
NA
NA

Attributes by original data submitter

Sample

source_name
Treg cells from FoxP3WT:hCD2 mice
strain
C57BL/6J
tissue
lymph nodes and spleen cells
cell type
CD4(+)hCD2(+) Treg cells
sorted from
FoxP3WT:hCD2 mice
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Magnetically sorted hCD2(+) WT and A384T Treg cells (approx. 1x10e7 cells per reaction, prepared in triplicates) were fixed for 30 min at room temperature with 1% formaldehyde, washed with PBS, and stored at -80 °C. Cells were resuspended in lysis buffers and sonicated for solubilization and shearing of crosslinked DNA. The cell extract was incubated overnight at 4 °C with 4 µg of the anti-FoxP3 antibodies and then with Dynabeads protein G (Dynal). Bound complexes were washed and eluted from the magnetic beads by heating at 65°C with occasional vortexing and crosslinking was reversed by overnight incubation at 65°C. The immunoprecipitated DNA and whole-cell extract input DNA were treated with RNase A and proteinase K, purified using MinElute PCR purification kit (QIAGEN) ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (NEB) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
21748766
Reads aligned (%)
99.0
Duplicates removed (%)
15.5
Number of peaks
483 (qval < 1E-05)

mm9

Number of total reads
21748766
Reads aligned (%)
98.7
Duplicates removed (%)
15.6
Number of peaks
516 (qval < 1E-05)

Base call quality data from DBCLS SRA