Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Natural Killer T-Cells
MeSH Description
A specific natural killer T-cell subtype that expresses an invariant T-cell receptor alpha-chain.

Attributes by original data submitter

Sample

source_name
Natural Killer cell
tissue
Natural Killer cell
origin
Peripheral blood
treatment
DMSO
antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, NEBNext Ultra DNA library prep kit for illumina (Cat #: E7420L) was used to prepare libraries. For ATAC-seq, transposed DNA was amplified using PCR ChIP and ATAC libraries were prepared for sequencing using standard NEB protocols designed for Illumina sequencing. Libraries were sequenced on the Illumina Nextseq following the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
28334397
Reads aligned (%)
94.4
Duplicates removed (%)
11.9
Number of peaks
26005 (qval < 1E-05)

hg19

Number of total reads
28334397
Reads aligned (%)
93.9
Duplicates removed (%)
12.7
Number of peaks
26071 (qval < 1E-05)

Base call quality data from DBCLS SRA