Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
sax-3

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_ZK377.2_GFP_L4_rep2_GFP_TGCT
strain
OP355(official name : OP355 genotype : unc-119(ed3) III; wgIs355(ZK377.2::TY1 EGFP FLAG; unc-119(+)) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Dresden using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The sax-3::EGFP fusion protein is expressed in the correct sax-3 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the sax-3 transcription factor. The sax-3 gene is encoded by the ZK377.2 CDS. made_by : R Waterston )
developmental stage
L4
genotype
unc-119(ed3) III; wgIs355(ZK377.2::TY1 EGFP FLAG; unc-119(+))
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM1076680: Snyder_ZK377.2_GFP_L4_rep2_GFP_TGCT
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
6721950
Reads aligned (%)
56.5
Duplicates removed (%)
11.9
Number of peaks
3097 (qval < 1E-05)

ce10

Number of total reads
6721950
Reads aligned (%)
56.5
Duplicates removed (%)
11.9
Number of peaks
3094 (qval < 1E-05)

Base call quality data from DBCLS SRA