Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Larvae
Cell type
L2
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_F45C12.2_Input_L2_rep2_TGCT
strain
OP212(official name : OP212 genotype : unc119(ed3); wgIs212(F45C12.2:TY1 EGFP FLAG; unc119) outcross : 3 transgene : F45C12.2 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The F45C12.2::EGFP fusion protein is expressed in the correct F45C12.2 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the F45C12.2 transcription factor. made_by : Mihail Sarov )
developmental stage
L2
genotype
unc119(ed3);wgIs212(F45C12.2:TY1 EGFP FLAG;unc119)
Sex
hermaphrodite
transgene
F45C12.2

Sequenced DNA Library

library_name
GSM1076654: Snyder_F45C12.2_Input_L2_rep2_TGCT
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
5831076
Reads aligned (%)
19.1
Duplicates removed (%)
1.8
Number of peaks
142 (qval < 1E-05)

ce10

Number of total reads
5831076
Reads aligned (%)
19.1
Duplicates removed (%)
1.8
Number of peaks
141 (qval < 1E-05)

Base call quality data from DBCLS SRA