Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mitotic Input
cell line
E14
cell type
Embryonic stem cells (ESCs)
cell subtype
mitotically purified ESCs

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated DNA from formaldehyde-fixed cells was pulled down with anti-Sox2 antibody. Libraries were prepared with NEBNext ChIP-seq Library Prep Master Mix kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
49277639
Reads aligned (%)
96.7
Duplicates removed (%)
55.5
Number of peaks
680 (qval < 1E-05)

mm9

Number of total reads
49277639
Reads aligned (%)
96.4
Duplicates removed (%)
55.6
Number of peaks
680 (qval < 1E-05)

Base call quality data from DBCLS SRA