Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
mESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cell line_input_no treatment
cell line
V6.5
antibody
none (input)
cell type
ESC-derived NPCs
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde. Cell lysates were obtained from sonicated nuclei and protein-DNA complexes were isolated with indicated antibodies. Libraries were prepared with KAPA LTP kit according to manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
12966326
Reads aligned (%)
92.5
Duplicates removed (%)
11.1
Number of peaks
350 (qval < 1E-05)

mm9

Number of total reads
12966326
Reads aligned (%)
92.3
Duplicates removed (%)
11.2
Number of peaks
344 (qval < 1E-05)

Base call quality data from DBCLS SRA