Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AFF1

Cell type

Cell type Class
Blood
Cell type
Pre-B cell leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
SEM(MLL-AFF1)
cell line
B cell precursor leukemia
drug treatment
IRAK4 inhibitor
chip antibody
homemade AFF4NT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using Trizol and QIAGEN RNeasy kit. DNA after ChIP protocol was isolated using QIAGEN PCR purification kit. ChIP-sequencing libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250- 450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit. RNA-sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Preparation Kit with Ribo-Depletion. Input RNA quality was validated using the Agilent RNA 6000 Nano Kit. 200ng-1 µg of total RNA was used as starting material. Libraries were validated using the Agilent DNA 1000 Kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
31076104
Reads aligned (%)
90.2
Duplicates removed (%)
6.8
Number of peaks
920 (qval < 1E-05)

hg38

Number of total reads
31076104
Reads aligned (%)
91.7
Duplicates removed (%)
5.9
Number of peaks
743 (qval < 1E-05)

Base call quality data from DBCLS SRA