Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

Blood

cell type

Resting TCM CD4 purified cells

chip antibodies

H3K4me1 (Abcam ab8895)

biowardrobe ids

395

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8-1%% formaldehyde for 8 min at RT and DNA was sonicated using Covaris or probe sonicators. RNA libraries for single read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

10544734

Reads aligned (%)

99.0

Duplicates removed (%)

7.4

Number of peaks

3490 (qval < 1E-05)

hg19

Number of total reads

10544734

Reads aligned (%)

98.7

Duplicates removed (%)

7.6

Number of peaks

3553 (qval < 1E-05)