Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Blood
cell type
Resting TCM CD4 purified cells
chip antibodies
H3K4Me3 (Millipore,17-614)
biowardrobe ids
479

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8-1%% formaldehyde for 8 min at RT and DNA was sonicated using Covaris or probe sonicators. RNA libraries for single read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
8130371
Reads aligned (%)
94.5
Duplicates removed (%)
51.3
Number of peaks
14065 (qval < 1E-05)

hg19

Number of total reads
8130371
Reads aligned (%)
94.0
Duplicates removed (%)
52.2
Number of peaks
14116 (qval < 1E-05)

Base call quality data from DBCLS SRA