GSM2370637: Naïve RNA Polymerase II; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Blood
cell type
Resting naive CD4 purified cells
chip antibodies
RNA Polymerase II (Active Motif, 4H8)
biowardrobe ids
881
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8-1%% formaldehyde for 8 min at RT and DNA was sonicated using Covaris or probe sonicators. RNA libraries for single read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).