Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
Hematopoietic Stem Cells
MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by original data submitter

Sample

source_name
Human MSCiPS1-derived 5F hematopoietic cells
tissue
Hematopoietic cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq analysis using the Illumina NextSeq500, 10-20 ng of ChIP DNA was processed for library generation using the NEBNext ChIP-seq Library Prep Master Mix following the manufacturer’s protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
15605335
Reads aligned (%)
93.1
Duplicates removed (%)
12.8
Number of peaks
11853 (qval < 1E-05)

hg19

Number of total reads
15605335
Reads aligned (%)
92.8
Duplicates removed (%)
13.3
Number of peaks
11800 (qval < 1E-05)

Base call quality data from DBCLS SRA