GSM2370659: input DNA WT rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD8+ T cell
strain
C57BL/6
subtype
Ezh2 fl/fl Cd4wt
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The C1 Single-Cell Auto Prep System (Fluidigm) was used to perform whole transcriptome amplification (WTA) of up to 96 single cells simultaneously. After cell isolation, FACS sorted 2.5 x 105 to 2 x 106 P14 CD8+ T cells were loaded onto the C1 Single-Cell Auto Prep mRNA Array IFC for single-cell capture on chip. Live/dead stain (Invitrogen) was included to exclude dead cells. Viable single cells captured on chip were manually imaged. Cell lysis and RT-PCR were performed on chip. SMARTer chemistry (Clontech) WTA was performed according to the manufacturer’s instructions. Illumina Nextera XT single-cell complementary DNA (cDNA) libraries were generated according to the manufacturer’s instructions (Illumina). Quality control measures of the single-cell cDNA libraries were performed on the 2100 Bioanalyzer (Agilent Technologies), Qubit 3.0 Fluorometer (Thermo Fisher Scientific), and MiSeq Sequencing System (Illumina). Single-cell cDNA libraries were sequenced (paired-end 100 or single-end 100) on the HiSeq2500 Sequencing System at the UCSD Institute for Genomics Medicine (IGM) Center.