GSM2367735: Input K562 NHS; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
K562 cells
cell line
K562
treatment
Non-treated (NHS)
antibody
no antibody, fragmented total chromatin
antibody_epitope
--
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After tretments, the cells were cross-linked with 1% fromaldehyde, quenched with glycine, washed twice with PBS and flash-frozen. The ChIP-experiments were conducted as described for GSE43579 (Vihervaara et al., Transcriptional response to stress in the dynamic chromatin environment of cycling and mitotic celss, 2013, PNAS, 110: 36, E3388-3397). The libraries were generated with Nextera DNA Sample Preparation kit (Illumina). Paired-end sequencing (HiSeq PE100) was performed with Illumina.