Antigen

Antigen Class

Histone

Antigen

H4K5K8K12K16ac

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562 cells

cell line

K562

treatment

Heat shocked (HS30)

antibody

Upstate 06-866

antibody_epitope

Recognizes H4 acetylation at lysines 5, 8, 12 and 16.

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After tretments, the cells were cross-linked with 1% fromaldehyde, quenched with glycine, washed twice with PBS and flash-frozen. The ChIP-experiments were conducted as described for GSE43579 (Vihervaara et al., Transcriptional response to stress in the dynamic chromatin environment of cycling and mitotic celss, 2013, PNAS, 110: 36, E3388-3397). The libraries were generated with Nextera DNA Sample Preparation kit (Illumina). Paired-end sequencing (HiSeq PE100) was performed with Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

27315574

Reads aligned (%)

90.9

Duplicates removed (%)

4.3

Number of peaks

28275 (qval < 1E-05)

hg19

Number of total reads

27315574

Reads aligned (%)

90.5

Duplicates removed (%)

4.3

Number of peaks

27964 (qval < 1E-05)