Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

Human primed PSCs and human extended PSCs, input

cell line

H1, H1-EPS

cell type

Human primed pluripotent stem cells and human extended pluripotent stem cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq library was prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

37857225

Reads aligned (%)

87.8

Duplicates removed (%)

0.3

Number of peaks

1210 (qval < 1E-05)

hg19

Number of total reads

37857225

Reads aligned (%)

87.0

Duplicates removed (%)

0.5

Number of peaks

614 (qval < 1E-05)