Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2A

Cell type

Cell type Class
Blood
Cell type
Bone marrow mononuclear cells
NA
NA

Attributes by original data submitter

Sample

source_name
Fetal bone marrow
cell type
Mononuclear cells from second trimester human fetal bone marrow
chip antibody
MLL1 (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
40492624
Reads aligned (%)
174.5
Duplicates removed (%)
6.2
Number of peaks
49552 (qval < 1E-05)

hg38

Number of total reads
40492624
Reads aligned (%)
176.0
Duplicates removed (%)
6.1
Number of peaks
49026 (qval < 1E-05)

Base call quality data from DBCLS SRA