Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Blood

Cell type

Bone marrow mononuclear cells

NA

NA

Sample

source_name

Fetal bone marrow

cell type

Mononuclear cells from second trimester human fetal bone marrow

chip antibody

MLL1 (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

40492624

Reads aligned (%)

93.7

Duplicates removed (%)

6.1

Number of peaks

49026 (qval < 1E-05)

hg19

Number of total reads

40492624

Reads aligned (%)

93.0

Duplicates removed (%)

6.2

Number of peaks

49552 (qval < 1E-05)