Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Blood

Cell type

Cord blood mononuclear

NA

NA

Sample

source_name

Cord blood

cell type

Mononuclear cells from full-term cord blood

chip antibody

MLL1 (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

35738497

Reads aligned (%)

172.4

Duplicates removed (%)

4.8

Number of peaks

24493 (qval < 1E-05)

hg38

Number of total reads

35738497

Reads aligned (%)

174.1

Duplicates removed (%)

4.6

Number of peaks

24650 (qval < 1E-05)