Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AFF1

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
L826
cell type
Presentation ALL patient sample engrafred in mice and harvested from spleen
chip antibody
AF4 (Abcam, ab31812)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
34092829
Reads aligned (%)
89.8
Duplicates removed (%)
4.6
Number of peaks
1012 (qval < 1E-05)

hg19

Number of total reads
34092829
Reads aligned (%)
89.1
Duplicates removed (%)
4.7
Number of peaks
913 (qval < 1E-05)

Base call quality data from DBCLS SRA