Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Leukemia cell line (SEM)
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
57605345
Reads aligned (%)
93.5
Duplicates removed (%)
1.0
Number of peaks
3550 (qval < 1E-05)

hg19

Number of total reads
57605345
Reads aligned (%)
92.7
Duplicates removed (%)
1.3
Number of peaks
2866 (qval < 1E-05)

Base call quality data from DBCLS SRA