Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Liver
Cell type
HuH-7
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HuH7 cells
treatment
1
antibody
p65 (Santa Cruz, C-20, sc-372)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP lysis buffer (1% SDS, 10mM EDTA, 50mM Tris pH 8.1, 1mM PMSF, Roche protease inhibitor mix), followed by IP and purification of DNA Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
22495753
Reads aligned (%)
84.0
Duplicates removed (%)
12.2
Number of peaks
1634 (qval < 1E-05)

hg38

Number of total reads
22495753
Reads aligned (%)
85.8
Duplicates removed (%)
11.0
Number of peaks
1611 (qval < 1E-05)

Base call quality data from DBCLS SRA