Antigen

Antigen Class

TFs and others

Antigen

SPI1

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP-1_1,25(OH)2D3_24hr_PU.1_ChIP

cell line

THP-1

cell type

human monocytic cell line

treated with

100nM VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)

chip antibody

PU.1 (Santa Cruz sc-22805 X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

proteins were crosslinked with formaldehyde to DNA, then PU1-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

28528380

Reads aligned (%)

84.4

Duplicates removed (%)

14.6

Number of peaks

116943 (qval < 1E-05)

hg38

Number of total reads

28528380

Reads aligned (%)

85.4

Duplicates removed (%)

14.0

Number of peaks

117212 (qval < 1E-05)