Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP-1_1,25(OH)2D3_24hr_PU.1_ChIP
cell line
THP-1
cell type
human monocytic cell line
treated with
100nM VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)
chip antibody
PU.1 (Santa Cruz sc-22805 X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
proteins were crosslinked with formaldehyde to DNA, then PU1-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
27213818
Reads aligned (%)
88.2
Duplicates removed (%)
10.8
Number of peaks
99212 (qval < 1E-05)

hg38

Number of total reads
27213818
Reads aligned (%)
89.3
Duplicates removed (%)
10.2
Number of peaks
99357 (qval < 1E-05)

Base call quality data from DBCLS SRA