GSM2359984: THP-1, PU.1 24 h EtOH rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SPI1
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
THP-1_EtOH_24hr_PU.1_ChIP
cell line
THP-1
cell type
human monocytic cell line
treated with
EtOH (vehicle) for 24hrs
chip antibody
PU.1 (Santa Cruz sc-22805 X)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
proteins were crosslinked with formaldehyde to DNA, then PU1-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol