Antigen

Antigen Class

TFs and others

Antigen

Was

Cell type

Cell type Class

Blood

Cell type

Thymocytes

MeSH Description

HEMATOPOIETIC PROGENITOR CELLS that have migrated to the THYMUS where they differentiate into T-LYMPHOCYTES. Thymocytes are classified into maturational stages based on the expression of CELL SURFACE ANTIGENS.

Sample

source_name

WASp thymus I296T sample

cell type

thymocytes

tissue

thymus

strain

C57BL/6

chip antibody

WASp F-8 (Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and WASp-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

15526660

Reads aligned (%)

98.5

Duplicates removed (%)

7.4

Number of peaks

190 (qval < 1E-05)

mm9

Number of total reads

15526660

Reads aligned (%)

98.5

Duplicates removed (%)

7.5

Number of peaks

195 (qval < 1E-05)