Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Liver
Cell type
HepaRG
NA
NA

Attributes by original data submitter

Sample

source_name
IP_STAT3_OL, HepaRG
cell line
hepatoma HepaRG cells
agent
sodium oleate
chip antibody
Phospho-Stat3 (Tyr705) XP #9145 Cell Signaling

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated from the nuclei of formaldehyde cross-linked cells, sonicated and immunoprecipated with anti-phospho-STAT3 antibody. ChIP-Seq libraries were constructed from 10ng immunoprecipitated or original input chromatin using the ChIP-Seq Sample Prep Kit from Illumina, following the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
112052983
Reads aligned (%)
1.9
Duplicates removed (%)
98.1
Number of peaks
411 (qval < 1E-05)

hg38

Number of total reads
112052983
Reads aligned (%)
2.8
Duplicates removed (%)
97.2
Number of peaks
493 (qval < 1E-05)

Base call quality data from DBCLS SRA