Antigen

Antigen Class

TFs and others

Antigen

STAT3

Cell type

Cell type Class

Liver

Cell type

HepaRG

NA

NA

Sample

source_name

IP_STAT3_vehicle, HepaRG

cell line

hepatoma HepaRG cells

agent

control

chip antibody

Phospho-Stat3 (Tyr705) XP #9145 Cell Signaling

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was isolated from the nuclei of formaldehyde cross-linked cells, sonicated and immunoprecipated with anti-phospho-STAT3 antibody. ChIP-Seq libraries were constructed from 10ng immunoprecipitated or original input chromatin using the ChIP-Seq Sample Prep Kit from Illumina, following the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg19

Number of total reads

55436318

Reads aligned (%)

2.0

Duplicates removed (%)

98.0

Number of peaks

77 (qval < 1E-05)

hg38

Number of total reads

55436318

Reads aligned (%)

2.8

Duplicates removed (%)

97.2

Number of peaks

42 (qval < 1E-05)