GSM2359485: RT112 8 Input combo BGJ398 plus THZ1 [lab: Hammerman-DFCI]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
RT-112
Primary Tissue
Urinary Bladder
Tissue Diagnosis
Carcinoma Transitional Cell
Attributes by original data submitter
Sample
source_name
Urinary bladder carcinoma
cell line
RT112
chip antibody
None
young_id
20160513_4702
tissue
bladder
drug
THZ1 / BGJ
concentration
100nM / 1 uM
cell number
50 million
treatment duration
48h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.