Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

Adenocarcinoma

cell line

A549

chip antibody

Brd4

antibody manufacturer

Bethyl

antibody catalog number

A301-985A100

young_id

20160509_4674

tissue

Lung

drug

THZ1 / GSK1120212

concentration

150nM / 200 nM

cell number

50 million

treatment duration

48h

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

31921394

Reads aligned (%)

90.2

Duplicates removed (%)

5.9

Number of peaks

1669 (qval < 1E-05)

hg38

Number of total reads

31921394

Reads aligned (%)

92.0

Duplicates removed (%)

4.8

Number of peaks

1704 (qval < 1E-05)